Consuming functional foods and drinks such as kombucha tea is believed to give many beneficial effects for promoting immunity and preventing cancer. This study was carried out to investigate hair growth potential of kombucha black tea and chamomile tea with and without methylsulfonylmethane (MSM) in vivo using Balb/c mice. The mice were divided into eight groups; minoxidil 2% as the positive control group, distilled water as the negative control group, black tea and chamomile tea as the control group, and the test groups which include kombucha fermented in black tea with and without MSM, kombucha fermented in chamomile tea with and without MSM respectively. The preparations were applied topically to the mice and observed for 30 days. Hair growth initiation time was observed for all groups, the results showed that treatment with kombucha/black tea with MSM reduced the time for hair growth initiation by 7 days. Hair density was assessed and the results indicated that there was a significant enhancement in hair density approximately 70% (P<0.0001) after applying kombucha/ black tea with MSM. Hair length were measured in two intervals the 15th and 30th day; the results showed a significant elongation of hair with p-value ? 0.0001 for the mice group that was treated with kombucha black tea with MSM. From the current study, we can conclude that kombucha fermented in black tea with MSM can be considered a natural and effective alternative therapy for hair loss, in addition to a cost-effective treatment with fewer side effects compared to synthetic drugs.