Ferrous sulfate and Folic acid have been widely used for the treatment of iron deficiency such as in anemia or pregnancy. Because they are ingested by oral they are leading to many of complication, for instance, GI disturbances (constipation, nausea, and unpleasant taste), and dangerously high blood pressure, severe headache, blurred vision and confusion with anxiety if taken as parenteral route. In the other hand, they are also interacting with numerous drugs, food and minerals). In this study, four FeSO4 µEm and 8 folic acid µEm systems were developed for transdermal application purpose to overcoming these problems resulted from Oral and parenteral routes. These W/O µEms are prepared by nonionic surfactants (Span20 & Tween80), IPM as oil phase, and water as aqueous phase. The µEms for both FeSO4 and Folic acid were characterized for their droplet sizes, zeta potential, pseudo-ternary phase diagram, and rheological properties. Moreover, the FTIR used to prove the encapsulation of folic acid in the µEm. The investigation demonstrate that FeSO4 and Folic acid µEms are correspond to the characteristics and properties of the µEm in literature. In this study, although, the most Microemulsions prepared have ideal characters to the Microemulsions properties written in literature. The results are shows that a significant variance between droplet size, Zeta potential, rheological properties and characterization of Microemulsions. The surfactants used in this study shows a factor that may play a role in preparation of those Microemulsions, and also the ratio of these surfactants have an important function to produce an ideal Microemulsion.