Parkinson's disease (PD) is caused by dopamine (DA)-containing neuron degeneration in the substantia nigra pars compacta (SNpc). Although the brain's neuroinflammatory response has long been thought to play a role in the etiology of this neurological illness, the mechanism remains unknown. Acetyl-L-Carnitine (ALC), which has antioxidant and anti-inflammatory effects, may be beneficial for PD neuroprotection. Approach: PD is a progressive disease, due to the continuous degradation of dopamine secreting cells due to several factors, including inflammation of the dopaminergic neurons. Since patients have a decreased response to PD medications, researchers are being conducted to find drugs or interventions that halt or reduce the disease's progression while protect the dopaminergic neuron. It has been demonstrated that Acetyl-L- carntine posses anti-oxidant and anti-inflammatory properties. Rationale: Parkinson's disease affects 2-3% of those over 65 and is the second most common neurodegenerative disease. We suggest investigating the effect of the Acetyl-L-Carnitine on the model of Parkinson's disease. Aim: This study aimed to investigate the possible effects of Acetyl-L-Carnitine on dopaminergic neurons from LPS-induced neurotoxicity in rats. Method: Thirty Male Wistar rats were placed into five groups randomly, each of which contains six rats. Animals anaesthetized, and received LPS (2 ?g/2 ?l) stereotaxically into left substantia nigra pars compacta (SNpc) to induce Parkinson's disease rat models. Rats administrated intraperitoneal Acetyl-L-Carnitine (100 mg/kg/day) before, and after LPS adminstrition for 14 days (2 ?g/2 ?l). The LPS induced lesions were evaluated behaviorally using the Apomorphine rotational test, and neurochemically by detecting dopamine amounts in the striatum, and brain-derived neurotrophic factor (BDNF) using an enzyme-linked immunosorbent assay (ELISA) kit. Results: Treatment with Acetyl-L-Carnitine (100 mg/kg/day) lowered tight contralateral rotations upon Apomorphine challenge and it increased DA levels slightly compared to the group injected with LPS only. Conclusion: Acetyl-L-Carnitine exerts a neuroprotective effect, improves the neurological impairments associated with PD, and appears to reduce neuroinflammation.