Contact lenses (CLs) are medical or cosmetic device, comfortable and more convenient alternative to eyeglasses. When microbes are inoculated onto CLs will result in eye infection because defense against microbial invasion in the anterior chamber of the eyes is weak due to the very poor blood supply. The present study, investigates microbial contamination of CL cases, solutions and rims of solution bottles and assess habits and practices of thirty CL wearers toward lens care to understand factors associated with CL units contamination through a structured questionnaire. Characteristics of grown colonies were determined through morphological and biochemical testing. Five (16.6%) CL units were found not contaminated. Eye redness after CLs wearing was almost statistically significant sign associated with CL units contamination (p=0.088). Sixty four isolates were obtained; the most prevalent bacteria were Pseudomonas spp.(25%), Staphylococcus spp. (21.9%), Serratia spp. (14.1%), and Acinetobacter spp. (6.2%). Two types of CL solutions (A and B) exceeded required level of bacterial reduction (3 log) and reached up to 5 log reduction. With regard to biofilms formation, after 4hr, tested solutions were able to reduce biofilm by more than 50% of all tested bacterial biofilms, regardless of the cleanness/dirtiness status. Using water to wash hands and CL cases has been incriminated for increasing contamination. Impurities in CL cases lead to reduced effectiveness of lens solutions. Value-added awareness of CL wearer should be improved by regular visit to eye care professional.