Background: Diabetes mellitus is one of the main causes of death around the world. Diabetic foot ulcer (DFU) is one of the most common complications that leads to increase morbidity, mortality, expenditure, and reduce the quality of life among patients have diabetes mellitus. Purposes: a) to assess nurses' diabetic foot ulcer knowledge, attitudes, and practices; b) to assess the relationships between nurses' demographic characteristics and DFU knowledge, attitude, and practices. Methods: A cross-sectional, descriptive design was used to guide the current study. Using convenience sampling, 256 nurses from nine governmental hospitals located in the central, north, and south of Jordan country, were participated in the current study. Based on international guidelines the researcher develop assessment tool entitled "The Knowledge, Attitudes, and Practices of Diabetic Foot Ulcer Questionnaire (KAP-DFUQ)", which contains 34 items. Descriptive statistics; a nonparametric test (chi-square test); binary logistic regression model; and the Pearson correlation coefficient were used to analyze and answer the different research questions. Results: Half of the participants had an inadequate level of knowledge (55%; n = 141), a negative attitude (52%; n = 132), and insufficient practices (50.4%; n = 129) toward DFU. The knowledge of nurses was affected by the following variables: age and working area (OR =.107, p=.040). Attitudes of nurses affected by the following variables: age group (41-50 years, OR = 3.891, p=.009) and training (OR =.576, p=.048). Furthermore, course training regarding of DFU had a significant positive effect on the attitudes of nurses (p = 0.042). The practices of nurses were affected by the following variables: gender (p = 0.040); and working area (OR = 5.712, p=.026). There was a significant positive correlation between nurses' knowledge and attitude scores (r = 0.384, p ? 0.01); in which furthermore, a positive correlation between nurses' knowledge and practice scores was reported (r = 0.313, p ? 0.01) as well as between nurses' practice and attitude scores (r = 0.572, p ? 0.01). Conclusion: Jordanian nurses had inadequate knowledge, negative attitudes, and insufficient practices of DFU. Healthcare organizations should have a national plan to encourage nurses to participate in theoretical and practical DFU training program, to improve their knowledge and practices of DFU. Keywords: diabetic foot ulcer, knowledge, attitude, practice, nurses, Jordan