This study aimed to measure the effect of psychological stress on the weight, hemoglobin (HbA1c) and fasting blood glucose (FPG) levels of pregnant women during pregnancy. This observational study was carried out on a sample of 385 pregnant women at 24-28 weeks (about 6 and 7 months) of gestation at Mafraq and Princess Badea hospitals. Demographic, medical and gynecological data were collected through direct interview with the participants as well as through review of patients records and the tool used to collect psychological data was the previously validated Depression, Anxiety, and Stress Scale (DASS). Results showed that most pregnant women had moderate to severe degree of stress, while mostly were with a moderate degree of depression and anxiety. In addition, more than half of the women showed abnormal FPG (50.6%), abnormal HbA1c level (56.4%) and around three quarter had abnormal weight gain (73.2%). A strong association between the psychological stress (DASS) and some demographic and gynecological characteristics were found (p-value <0.05). Abnormal depression and anxiety scores were associated with gestational diabetes development (p=0.015 and p=0.010, respectively) while neither depression, nor anxiety, nor stress was associated with abnormal weight gain during pregnancy. The study therefore recommended adopting strategies to assess depression and anxiety in women prior to pregnancy in order to avoid their effect on glucose levels on mothers and fetus during pregnancy and afterbirth. Keywords: Stress, Anxiety, Depression, Gestational Diabetes, Gestational weight gain