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2018 Publications

International Journal of Applied
Engineering Research

Volume 13, Number 7 (2018) pp. 4693-
4701

Development and validation of an RP-HPLC method for simultaneous
determination of sorbic acid, benzoic acid, and natamycin in domestic yogurt in

Jordan

Ala’ Yahya Sirhan, Lukman Bola Abdulra'uf, Ahmad Mostafa, Ahmad Talhouni, Yousef Al-Ebini

A method for the simultaneous determination and analysis of sorbic acid, benzoic acid, and
natamycin in yogurt products, based on the use of the high-performance liquid
chromatography with a UV detector (HPLC–UV) has been developed. This method excludes
the employment of complex procedures, time-consuming and labor-intensive pre-treatment
processes. Separation of the sorbic acid, benzoic acid, and natamycin with higher selectivity
and sensitivity, and within five minutes was performed by a C8 150 mm × 4.6 mm x 3 μm
column (Hypersil™ BDS) at 20oC. The mobile phase was a mixture of trifluoroacetic acid 0.1
% in water containing 100 mM sodium acetate, trifluoroacetic acid 0.1 % in acetonitrile, and
trifluoroacetic acid 0.1 % in tetrahydrofuran (65:25:10 (%, v/v)). Using the mobile phase as an
extraction mixture gave recoveries in the range of 83.0-110.2% at spike levels of 2.5–80.0
μg/kg with relative standard deviations (RSDs) lower than 10%. The intra-day precision and
inter-day precision results were in the range of 5.3–6.7% and 7.6–9.2%. Additionally, the
limits of detection (LOD) were 0.24, 0.39, and 0.61 mg/L and the limits of quantification
(LOQ) were 0.80, 1.3, and 2.0 mg/L for sorbic acid, benzoic acid, and natamycin, respectively.
https://www.semanticscholar.org/paper/Development-and-validation-of-an-RP-HPLC-method-for-
Sirhan-Abdulra%E2%80%99uf/66274d1282048c4b5bba681c3efe9b40dce9b204

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